Year : 2023 | Volume
: 23 | Issue : 1 | Page : 10--16
Comparing 16S rRNA gene similarity with simple polar lipids profiling amongst Salmonella isolates
I M T Fadlalla1, ME Hamid2, A G A Rahim4, E D M Elamin3
1 Department of Biomedical Sciences, College of Veterinary Medicine, Sudan University of Science and Technology, Khartoum, Sudan; Department of Biochemistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
2 Department of Microbiology, College of Medicine, King Khalid University, Abha, Saudi Arabia; Department of Biochemistry, Faculty of Veterinary Medicine, University of Khartoum, Khartoum, Sudan
3 Department of Microbiology, Animal Resources Research Corporation, Khartoum, Sudan
Background and Objectives: Polar lipids and the 16S rRNA gene have a significant role in taxonomic characteristics. The study's objective is to determine the potential relationship between simple migration distances (mm) of polar lipids and Salmonella's 16S rRNA gene similarity.
Materials and Methods: Based on 16S RNA sequences and simple thin-layer chromatography migration distances (mm) of polar lipids, allowed to compare the various Salmonellae species, and apply it to examine the variability and estimate bacterial similarities.
Results: The chromatography migration distance analysis revealed 3–4 spots of polar lipids. The polar lipids revealed two denser spots, the most abundant lipids, between 10 and 28 mm. The other two low-density spots of migration distance ranged in size from 23 to 25 mm. Between 99.4% and 100% of the three Salmonella isolates and other Salmonella species exhibited 16S rRNA similarities. One strain had a similarity of 98.9%. These findings demonstrated the nearly identical 16S rRNA gene sequences and polar lipids profile of the isolates.
Conclusions: This study has concluded that all Salmonella species share similarities in both the polar lipid profiles and the 16S rRNA genes. The study validates the utility of coupling of polar lipids and 16S rRNA gene sequencing as useful tools for taxonomic differentiation and epidemiological tracing of Salmonella.
Prof. I M T Fadlalla
College of Veterinary Medicine, Sudan University of Science and Technology, P.O. Box 204, Khartoum
|How to cite this article:|
Fadlalla I M, Hamid M E, Rahim A G, Elamin E D. Comparing 16S rRNA gene similarity with simple polar lipids profiling amongst Salmonella isolates.Niger J Health Sci 2023;23:10-16
|How to cite this URL:|
Fadlalla I M, Hamid M E, Rahim A G, Elamin E D. Comparing 16S rRNA gene similarity with simple polar lipids profiling amongst Salmonella isolates. Niger J Health Sci [serial online] 2023 [cited 2023 Sep 26 ];23:10-16
Available from: http://www.https://chs-journal.com//article.asp?issn=1596-4078;year=2023;volume=23;issue=1;spage=10;epage=16;aulast=Fadlalla;type=0